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Tivation of the Spc1 MAPK cascade. Therefore we further characterized the heteromer interaction between Wis4 and Win1 by testing truncated Wis4 fragments for their interaction with Win1 in a yeast two-hybrid assay. We found that the N-terminal region (amino acid residues 1?00) of Wis4 binds Win1 (Figure 4A). This Wis4(1300) fragment was subsequently tested for interaction with a series of truncate
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